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Overview and Learning Objectives
Central Concepts
Benchmarks and Standards
Activity Credits

Chromatography (a multi-page technical education activity)

Interactive, scaffolded model

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Overview and Learning Objectives

Students learn about the phases of molecular separation, and then experiment with paper and ion chromatography.

Students will be able to:

  • identify the role of the mobile and stationary phases in the separation process;
  • compare paper and ion chromatography;
  • manipulate hydrophobic and hydrophilic characteristics of the mobile and stationary phases in order to compare results.

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Central Concepts

Key Concept:

Chromatography uses differences in weak attractions among molecules to effect molecular separation.

Additional Related Concepts

Molecular Biology

  • Molecular separation
  • Protein


  • Hydrophobicity
  • van der Waals


  • Emergent Properties

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Benchmarks and Standards


  • THE PHYSICAL SETTING: FORCES OF NATURE - Electromagnetic forces acting within and between atoms are vastly stronger than the gravitational forces acting between the atoms (Full Text of Standard)

  • THE PHYSICAL SETTING: FORCES OF NATURE - There are two kinds of charges-positive and negative. (Full Text of Standard)


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Activity Credits

Created by CC Project: Molecular Workbench using Molecular Workbench + Pedagogica

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Last Update: 12/07/2015 Maintainer: CC Web Team (webmaster@concord.org)
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These materials are based upon work supported by the
National Science Foundation under grant number DUE-0402553

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or recommendations expressed in this material are those of
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of the National Science Foundation.